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pentr nls actin r62d  (Addgene inc)


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    Structured Review

    Addgene inc pentr nls actin r62d
    Pentr Nls Actin R62d, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+nls+actin+r62d/pFLAG-CrmA+(Plasmid+%2311831)/pmc13036013-231-116-117
    Average 92 stars, based on 3 article reviews
    pentr nls actin r62d - by Bioz Stars, 2026-10
    92/100 stars

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    Construct:

    Article Title: Compartmentalized Cytoplasmic Flows Direct Protein Transport to the Cell’s Leading Edge
    Article Snippet: .. PaGFP-Actin was constructed by excising the color from PaGFP-C1 (gift from George Patterson) and EGFP-Actin , . mEmerald-LC-Myosin-N7 was a gift from Michael Davidson (Addgene no. 54146). mNeon LC-Myosin-N7 was constructed using the enzymatic digests described in the Supplementary Information. mEos3.2 ARC3 was constructed from EGFP-ARC3, a gift from Matt Welch (Addgene no. 8462) , and mEos3.2-N1 (Addgene no. 54525) using SmaI and Not I. mEos 2 Paxillin-22 was a gift from Michael Davidson (Addgene no, 57409). pENTR-NLS-actin-R62D (Addgene no. 11831) was used to construct the mEos3.2 actin-R62 plasmids using Sali-HF and BmbG1 to combine the mutated region of actin with existing mEos3.2 actin plasmids. ..

    Article Title: Compartmentalized cytoplasmic tradewinds direct soluble proteins
    Article Snippet: .. PaGFP-Actin was constructed by exchanging the color from PaGFP-C1 (gift from George Patterson, Addgene no. 11910) and egfp-Actin using BsPE1 and BamH1 , . mNeon LC-Myosin-N7 was constructed by exchanging color with mEmerald-LC-Myosin-N7 (gift from Michael Davidson, Addgene no. 54146) and mNeon Lifeact (Addgene no. 9887) using BsrGI and AgeI. mEos3.2 ARC3 was constructed by exchanging color with egfp-ARC3, a gift from Matt Welch (Addgene no. 8462) and mEos3.2-N1 (Addgene no. 54525) using SmaI and Not I. mEos2 Paxillin-22 (Addgene no. 57409) and mEos3.2 Lifeact-7 (Addgene no. 54696) were gifts from Michael Davidson. mEos3.2 Vinculin-N-21 was constructed by exchanging color with mEos2-vinculin-14 (Addgene 57438) and mEos3.2-N1 (Addgene no. 54525) and is available as (Addgene no. 6692). pENTR-NLS-actin-R62D (Addgene no. 11831) was used to construct the mEos3.2 actin-R62 plasmids using SalI-HF and BmbG1 to combine the mutated region of actin with existing mEos3.2 actin plasmids. ..



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